A series of novel NGS technologies have been developed for B cell repertoire analysis. Specifically, 5?-RACE PCR, reverse primers targeting constant regions, and 600bp long-read NGS are combined to capture B cell repertoires in an unbiased manner, which is key to repertoire profiling. A single-molecule barcoding strategy has been devised to reduce amplification noise and sequencing error. NGS data can be processed by the Antibodyomics pipeline to identify functional antibodies and dissect antibody lineages. These repertoire technologies are applicable to humans, macaques, rabbits, and mice.